IVIS® Spectrum System

Non-Invasive Quantitative 3D Molecular Optical Imaging System

IVIS® Spectrum, most trusted optical imaging system combines high throughput and full tomographic optical imaging in one platform. The system uses leading optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living small animals. Only system in the industry which offers true 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in anatomical context against a Digital Mouse Atlas or Co-registered with other tomographic technologies such as MR, CT or PET through the multimodality modules. Take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region using optimized set of high efficiency filters and spectral unmixing.

The instrument is equipped with 10 narrow band excitation filters and 18 narrow band emission filters that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. The spectral unmixing tools available only in perkin elmer IVIS instruments enables the researcher to separate signals from multiple fluorescent reporters within the same animal. You can choose from Perkin Elmer’s extensive portfolio of Invivo Imaging Fluorescent and Bioluminescent Cell Lines, Agents and Dyes to simplify your research.

Living Image® Software has been specially designed to simplify advanced and complex biological models by intuitively guiding the user through experiential setup and analysis. The imaging wizard option with a range of newly added probe library helps user design imaging settings and selects the right filter pair for fluorescence studies.

Key Features
  • True 3D full Tomographic optical imaging
  • High throughput
  • In vivo, in vitro and ex vivo
  • Exquisite sensitivity in Bioluminescence, Fluorescence, Chemiluminescence, Cerenkov Imaging
  • Full fluorescence tunability through the NIR Spectrum
  • Up to 18 filter sets
  • Transmission & Reflectance Imaging
  • NIST Absolute calibration
  • Compute Pure Spectrum spectral unmixing
  • Digital Mouse Atlas for Anatomical Reference
  • Deep Tissue Imaging & Precise X,Y Source location
  • Co-registration of 3D data with other 3D modalities such as PET/SPECT/MRI etc.

Instrument Specifications

  • Camera
  • CCD Size
  • Imaging Pixels
  • Pixel Size
  • Min. Detectable Radiance
  • Min. Field of View (FOV)
  • Max. Field of View (FOV)
  • Min. Image Pixel Resolution
  • Lens
  • Read Noise
  • Dark Current (Typical)
  • Excitation Fluorescence Filters
  • Emission Fluorescence Filters
  • Transillumination Stage
  • 3D Tomography Software
  • CCD Operating Temperature

Sensor Back-thinned, back-illuminated Grade 1 CCD
2.7 x 2.7 cm
2048 x 2048
> 85% 500-700 nm; > 30% 400-900 nm
13.5 microns
70 photons/s/sr/cm2
3.9 x 3.9 cm
23 x 23 cm
f/1 – f/8 1.5x, 2.5x, 5x, 8.7x magnifications
< 3 electrons for bin=1,2,4; < 5 electrons for bin=8,16
< 100 electrons/s/cm2
-90 °C

Hardware Specifications

  • Imaging Chamber Interior Size
  • Imaging System Space Requirement
  • Power Requirements
  • Stage Temperature
  • Heated Chamber
  • Gas Anesthesia Ports
  • Injector Ports

43 x 50 x 60 cm (W x D x H)
203 x 163 x 214 cm (W x D x H)
20 Amps for 120 VAC or 10 Amps for 230 VAC
20-40 °C
  • Oncology
  • Stem Cell Research
  • Infection Biology
  • Neurodegenerative Disorders
  • Parasitology
  • Virology
  • Crispr/Cas9 Applications
  • Inflammation Biology
  • Immunology Research
  • Plant Biology
  • many more
  • Living Image® Software

PerkinElmer's IVIS Spectrum Pre-clinical In Vivo Imaging System
In Vivo Bioluminescence Imaging on the IVIS Platform