MinION

Only portable real-time device for DNA and RNA sequencing.


Technology that offers:

  • Direct DNA/RNA sequencing
  • REALReal-time
  • No capital cost required
  • Ultra-long reads
  • Scalable to portable or desktop
  • 10 min library prep
  • High-fidelity
  • High yields for large genomes

Each consumable flow cell can now generate 10–20 Gb of DNA sequence data. Ultra-long read lengths are possible (hundreds of kb) as you can choose your fragment length. The MinION streams data in real time so that analysis can be performed during the experiment and workflows are fully versatile.

Features:

  • Pocket-sized, portable device for biological analysis
  • Up to 512 nanopore channels
  • Simple 10-min sample prep available
  • Real-time analysis for rapid, efficient workflows
  • Adaptable to direct DNA or RNA sequencing
  • MinIT available to support IT/software needs

The MinION weighs under 100 g and plugs into a PC or laptop using a high-speed USB 3.0 cable. No additional computing infrastructure is required. Not constrained to a laboratory environment, it has been used up a mountain, in a jungle, in the arctic and on the International Space Station.

Long reads –

  • Choose your read length: 5kb? >200kb? Longer?
  • Easier assembly, phasing
  • Covering repetitive regions

Real time –

  • Immediate access to data
  • Rapid time to result – move on
  • Rapid insight into whether status of sample

Easy, rapid prep –

  • 1D library prep: <10mins, 2 pots
  • Low cost of materials
  • De-skill prep

On demand –

  • Run different experiments in sequence on one flow cell
  • Barcode for even more samples
  • Run many experiments on one device

Accessible –

  • No capital cost
  • Easy install
  • No additional lab infrastructure requirements

Nanopore sequencing offers advantages in all areas of research:

  • Microbiology
  • Environmental research
  • Microbiome
  • Basic genome research
  • Human genetics
  • Cancer research
  • Clinical research
  • Plant research
  • Transcriptome analysis
  • Population genomics
  • Animal research

Whole genome sequencing

  • De novo assembly
  • Scaffolding and finishing
  • Variant analysis: structural variation
  • Variant analysis: SNVs, phasing
  • Resequencing
  • Targeted sequencing

  • Panels – amplicons, sequence capture, exome
  • Variant analysis: structural variation
  • Variant analysis: SNVs, phasing
  • 16S rRNA analysis
  • RNA sequencing

  • Splice variant analysis
  • Transcriptome / gene expression
  • Fusion transcript analysis
  • Metagenomics

  • Real-time, unbiased analysis of mixed samples
  • Epigenetics

  • Methylation
  • Histone modification
  • Non-coding RNA activity
  • MinKNOW

  • A Portable, Real-Time DNA/RNA Sequencing Device
    Sequencing DNA (or RNA) | Real-time, Ultra Long-Reads, Scalable Technology from Oxford Nanopore
    RNA Sequencing with Nanopore Technology