IVIS® Lumina III System

Gold Standard Benchtop In-Vivo small animal optical Imaging system

The IVIS® Lumina Series III platforms from PerkinElmer brings together years of leading optical imaging technologies into one easy to use and exquisitely sensitive bench-top system and is capable of imaging both full spectrum fluorescence and bioluminescence. The system includes a highly sensitive CCD camera, light-tight imaging chamber and complete automation and analysis capabilities. The system is equipped with 26 filters that can be used to image reporters that emit from green to near-infrared. Superior spectral unmixing can be achieved by Lumina III’s high resolution short cut off filters. Absolute calibration coupled with our Living Image® software affords you consistent and reproducible results independent of magnification and/or filter selection. This allows you to compare results from one IVIS® system to another, either within your organization or around the world. All Lumina III series instruments now incorporate PerkinElmer’s patented Compute Pure Spectrum (CPS) algorithm for spectral library generation software tools to ensure accurate autofluorescence removal, unmixing and fluorophore quantitation.

The Series III provides the highest degree of sensitivity of any optical imaging system on the market representing the gold standard for non-invasive small animal optical imaging sensitivity. This degree of sensitivity is achieved through the combination of optimized luciferase reporters, agents and unique platform technologies including an Ultra Cooled CCD USB Camera to -90 °C, low readout noise, dark current and exceptionally high quantum efficiency throughout the NIR spectrum.

An adjustable field of view from 5 – 12.5 cm and an optional 24 cm lens allows imaging of up to 5 mice or 2 medium size rats or zoom lens capability increasing the resolution to 35 μm at a field of view of 2.5 cm. The Lumina Series III can also accommodate petridishes or micro-titer plates for in vitro imaging. The system includes premium animal handling features such as a heated stage, gas anesthesia connections and ECG monitoring.

Features and Benefits:
  • Market trusted technology offering the fullest suite of leading imaging technologies, reagents and support
  • Exquisite sensitivity in bioluminescence
  • Full fluorescence tunability through the NIR spectrum
  • Compute Pure Spectrum spectral umixing for ultimate fluorescence sensitivity
  • Expandable system tailored to your workflow

Imaging System Components:

  • Camera Sensor
  • CCD Size
  • Imaging Pixels
  • Quantum Efficiency
  • Pixel Size
  • Minimum Detectable Radiance
  • Minimum Field of View (FOV)
  • Maximum Field of View (FOV)
  • Minimum Image Pixel Resolution
  • Read Noise
  • Dark Current (Typical)
  • Minimum Detectable Radiance
  • Lens
  • Illumination Source Extended NIR Range
  • Fluorescence Capability
  • Excitation Fluorescence Filters
  • Emission Fluorescence Filters
  • CCD Operating Temperature


Back-thinned, back-illuminated, cooled Grade 1 CCD
1.3 x 1.3 cm
1024 x 1024
>85% at 500 – 700 nm, >30% at 400 – 900 nm
13 microns
100 photons/s/sr/cm2
5 x 5 cm
12.5 x 12.5 cm (optional 24 x 24 cm)
50 microns
< 3 electrons for bin = 1, 2, 4; < 5 electrons for bin = 8, 16
< 120 electrons/s/cm2; or 2 x 10-4 electrons/s/pixel
100 photons/s/sr/cm2
f/.95 – f/16, 50 mm
150W Tungsten EKE

-90 °C

Hardware Specifications

  • Imaging System Space Requirement
  • Imaging Chamber Interior Dimension
  • Power Requirements
  • Stage Temperature
  • Computer (Minimum Specifications)

48 x 71 x 104 cm (W x D x H)
43 x 38 x 43 cm (W x D x H)
6A at 120V
20 – 40 °C
Quad Core 2.8 GHz, 4 GB RAM, 16X DVD+/-RW, 250 GB HD, 24” flat screen monitor
  • Oncology
  • Stem Cell Research
  • Infection Biology
  • Neurodegenerative Disorders
  • Parasitology
  • Virology
  • Cardiovascular studies
  • Pharmacokinetics/Pharmacodynamics studies
  • Crispr/Cas9 applications
  • Inflammation biology
  • Immunology research
  • Plant Biology
  • many more
  • Fluorescent agents, Dyes, Labeling Kits, and Nanoparticles
  • Bioluminescent oncology cell lines and bacterial created using luciferase (luc2) vector for utlra brite sensitivity
  • Bioluminescent substrates including D-Luciferin K+ salt and RediJect Luciferin in ready-to-use injectable format
  • Radioimaging reagents
  • Living Image® Software

Pre-Clinical In Vivo Imaging Solutions from PerkinElmer
In Vitro Bioluminescence Imaging on the IVIS Platform
In Vivo Bioluminescence Imaging on the IVIS Platform
In Vitro Fluorescence Imaging on the IVIS Platform