High Throughput Size Selection for Next-Gen Sequencing

PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing large-scale sequencing data.

The Pippin systems from SageScience allow for precise automated size selection of nucleic acid samples, sequencing libraries, and even protein samples. The Pippin HT excels at high-throughput applications and also works with smaller sample amounts. The Pippin HT sample sizing instrument follows to precisely size-select DNA samples and sequencing libraries for up to twenty-four samples per single run.

The Pippin HT agarose cassettes available in the Core and the size ranges in which samples can be sized: 0.75% from 6 kb up to 40 kb high -pass; 1.5% from 250 bp up to 1.5 kb; 2% from 100 bp up to 600 bp; For the the 2% cartridge selection intervals can be as small as 20 bp.

In addition to high-throughput usefulness, Tight (Narrowest fragment range based on a target size), Range (Set with start and end base pair values) and High-Pass (Collects sizes above a base pair threshold) protocols, PippinHT allows user to size select DNA fragment with Low-Pass (Collects pre-library nucleic acids below a threshold) protocol specificaly designed for this system. The PippinHT syestem provides, sample-to-sample reproducibility to streamlines research, higher sample quality and 24 sample capacity which meets high throughput sequencing demands.

  • Electrophoresis voltage 75V constant, 75V pulsed field
  • Optical detection 470 nm excitation, 525 nm emission
  • Power requirements 100-240 VAC, 2.5 A, 50-60 Hz
  • Weight 20 lbs / 9 kg
  • Dimensions (with lid open) 18h x 13w x 17d (in) | 46h x 33w x 64 d (cm)
  • The Pippin Prep instrument includes a monitor, keyboard, and mouse. A CPU is built-in, an external PC is not required
  • whole genome sequencing
  • miRNA library isolation
  • 3% agarose, 100-250bp
  • 2% agarose, 100-600bp
  • 1.5% agarose, 300bp-1.5kb
  • 0.75% agarose, high-pass size selection
  • PippinHT Software; inbuilt and supplied alongwith the instrument; easier to upgrade with latest versions freely available on Sage sciences website time to time.

About the PippinHT
PippinHT Installation Guide
Sample Prep for the PippinHT
How to Create a Protocol for PippinHT
Optical Calibration of the PippinHT
Preparing Cassettes for the PippinHT
Loading Samples on the PippinHT
Running the Pippin HT
How to Track Samples on the PippinHT