The Simoa HD-X Analyzer™ is the latest model of the fully automated Simoa bead-based immunoassay platform. Re-engineered, the platform’s ultra-sensitivity and dependability empower researchers with 1000x greater sensitivity than traditional immunoassays. Leveraging years of experience with the ground-breaking Simoa HD-1 analyzer, the HD-X delivers increased productivity, user flexibility, unparalleled sensitivity and best in class assay performance.

The Simoa bead-based technology isolates each bead-immunocomplex to allow for enzyme bound to the capture antibody to produce sufficient fluorescence in each well to be detectable, even when just a single molecule is present. As a result, the analyzer produces a map of the array—each of which contains more than 200,000 wells—where each well can be digitally analyzed as either “on” (containing a target molecule) or “off”. The proprietary Simoa algorithm then converts the measurement into a concentration which reaches femtomolar levels for most analytes. This revolutionary approach to digitalizing ELISA offers on average a 1000-fold improvement in sensitivity over current immunoassay techniques.

Key features include:

  • Ultra-sensitivity – Up to 1000x greater sensitivity than traditional immunoassays
  • Automation – Reproducibility and convenience of sample->answer workflow
  • Multiplexing – Measure up to 6 biomarkers in single sample at fg/ml concentrations
  • Flexibility – Supports both highthroughput and rapid result workflows
  • Efficiency – Precise control of assay conditions allows for use of stored assay calibration values
  • Menu – Fully compatible with 80+ assays also available on HD-1 as well as customized homebrew assays
  • Regulatory Compliance – Enables 21CFR Part 11 compliance with streamlined run reports and user management

Instrument Specifications

  • Throughput

  • Workflow
  • Total Assay Time
  • Hands on Time
  • Automation
  • Sample Input
  • Sample Volume
  • Instrument Footprint
  • Multiplex Capability
  • Assay Flexibility

3 Plates /8hour Shift
>300 data points per day
Batch (plates or tubes)
<2.5 hours per 96-well plate
Startup time <20 minutes
Full (sample in to data out)
96-well plate and tubes
1 μl * - 100 μl
135 x 60 x 160 cm (freestanding)
up to 6-plex
1, 2 or 3-step assays, with variable incubation time and number of wash steps
  • Oncology
  • Neurology
  • Cardiology
  • Infectious diseases
  • Immunology
  • Simoa Kit
  • Disc Kit
  • Conical well plates for Simoa
  • Sealing oil
  • System buffers

Simoa Technology