ONI

Every Molecule Counts – Now on Your Benchtop

Break the diffraction limit and visualize biology at ≤15 nm resolution with compact, powerful super-resolution systems from ONI.

About ONI

Oxford Nanoimaging (ONI) is a globally recognized innovator in single-molecule and super-resolution microscopy, headquartered in the UK.

Founded with a mission to democratize nanoscale imaging, ONI has transformed traditionally complex and infrastructure-heavy microscopy into compact, automated, and accessible benchtop systems.

Their platforms enable researchers to visualize, track, and quantify individual molecules in cells, tissues, nanoparticles, and biological systems.

Technology Overview

ONI systems are built on Single-Molecule Localization Microscopy (SMLM), enabling resolution far beyond conventional microscopy.

ONI Product Range:

• Aplo Scope:- High-throughput, multicolor super-resolution microscope with ≤15 nm resolution, 110 × 110 µm homogeneous FOV, and seamless switching between live-cell and super-resolution modes.
• Nanoimager:- The world’s first compact benchtop super-resolution microscope (≤20 nm resolution) for fixed/live-cell imaging with multiple imaging modes.
• Aplo Flow:- – Automated sample preparation and liquid-handling platform for ONI workflows, reducing hands-on time by up to 85%
• CODI Software: – Cloud-based collaborative analysis, storage, and sharing platform for single-molecule imaging data.
• ONI Kits: – Ready-to-use reagent kits for dSTORM training, EV profiling, and custom biomarker studies.

Supported Technologies:

• dSTORM
• PALM
• DNA-PAINT
• Single Particle Tracking (SPT)
• smFRET (molecular interaction studies)
• Modes : TIRF, HILO & Epifluorescence imaging

Key Capabilities:

• Resolution down to ≤15 nm (Aplo Scope)
• Multi-color imaging (up to 5 colors)
• Real-time molecule localization
• Live-cell + fixed-cell compatibility
• Seamless switching between imaging modes

Product Portfolio

Nanoimager

World’s first benchtop super-resolution microscope

• ≤20 nm resolution
• FOV: 50 × 80 µm
• Ultra-compact footprint (fits on any lab bench)
• Supports dSTORM, PALM, PAINT, SPT
• No optical table or dark room required

Ideal for academic labs, core facilities, Bio pharma and translational research

Aplo Scope

Simple. Engineered. Dynamic. Powerful.

• ≤15 nm super-resolution imaging
• Large homogeneous FOV: 110 × 110 µm
• 4-laser multiplexing (405, 488, 561, 640 nm)
• Up to 5-color imaging
• Live-cell to super-resolution switching (no reconfiguration)
• Temperature control up to 40°C
• Fully integrated with Aplo platform (fluidics + software)

Designed for high-throughput, automated, reproducible research

Aplo Platform (End-to-End Ecosystem)

• Aplo Flow – Automated sample preparation (reduces hands-on time by ~85%)
• CODI Software – Cloud-based acquisition, analysis & collaboration
• ONI Kits – Ready-to-use reagent kits for reproducible workflows

Complete workflow: Prepare → Image → Analyze → Quantify

Why ONI Stands Out/USP

• Benchtop system (no dark room / optical table)
• Ultra-high resolution (≤15 nm)
• Multi-modal imaging in one platform
• Large field-of-view for higher throughput
• Highly stable, no alignment required
• Integrated AI-powered software (CODI
• End-to-end ecosystem (hardware + reagents and kits+ software

Why Imperial Life Sciences

As the official channel partner for ONI in India, Imperial Life Sciences provides:

• Expert consultation for system selection
• Application-specific workflow design
• Installation & hands-on training
• Dedicated technical and service support
• Strong experience in cancer, EV, and molecular biology research

We help you translate nanoscale imaging into real biological insights.